thr 77 - An Overview

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Antibodies bound to the blocking peptide not bind to your epitope about the target protein. By evaluating the staining in the blocked antibody vs . the antibody on your own, you can see which staining is certain.

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This manual outlines even more achievable optimization of cellular and immunoassay parameters to be certain the very best effects are...

This information outlines further more attainable optimization of cellular and immunoassay parameters to guarantee the best possible final results are...

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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by each antibodies...

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Blocking peptides are peptides that bind particularly into the focus on antibody and block antibody binding. These peptide generally has the epitope recognized because of the antibody.

Target Data Cyclin E belongs to the remarkably conserved cyclin loved ones, whose users are characterized by a remarkable periodicity in protein abundance in the cell cycle. Cyclins purpose as regulators of CDK kinases. Different cyclins exhibit unique expression and degradation designs which add into the temporal coordination of each mitotic occasion. Cyclin E kinds a fancy with and capabilities to be a regulatory subunit of CDK2, whose activity is necessary for mobile cycle G1/S changeover. This protein accumulates at the G1-S phase boundary and it is degraded as cells progress by means of S period.

Overexpression of the gene has long been observed in many tumors, which results in chromosome instability, and thus may possibly contribute to tumorigenesis. This protein was observed to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a significant position in selling cell-cycle development in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are already here described. Two additional splice variants were described but detailed nucleotide sequence info just isn't however obtainable.

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